Fungal infections of the scalp, such as dermatophytosis caused by Pityrosporum ovale, are a common health problem that cancause discomfort and hair damage. Conventional treatment often has limitations, including the problem of antifungal resistance. African leaves (Vernonia amygdalina) are known to contain bioactive compounds such as flavonoids, saponins, and tannins thathave significant antifungal activity. This study aims to develop and evaluate hair tonic preparations based on African leaf extractas an alternative treatment for fungal infections on the scalp. This study used an experimental method by formulating a hair tonic containing African leaf extract in varying concentrations (1%, 2%, and 3%). The process of making extracts is carried out throughmaceration using 96% eth anol. Evaluation of preparations includes physical tests (organoleptic, pH, viscosity, and homogeneity).The antifungal activity test against Pityrosporum ovale was carried out using the agar diffusion method (continuous diffusion). The test results data were statistically analyzed, including normality and homogeneity tests, followed by ANOVA and post hoc tests if needed. The results showed that the formulated hair tonic preparations met the expected physical test criteria, such as organoleptics exhibiting liquid form, deep green color, and distinctive smell of African leaves. The preparation also shows good homogeneity without undispersed particles. The pH test shows a value that corresponds to the standard hair tonic (pH 3-7). The viscosity of the preparation also meets the set standards (1-5 mPas). Skin irritation tests show that the African leaf extract hair tonic formulation is safe to use topically and does not cause a primary irritant reaction on rabbit skin. In antifungal activity tests, African leaf extract hair tonic preparations were effective in inhibiting the growth of Pityrosporum ovale. Nonetheless, the effectiveness of antidandruff varies between formulations, with formulation 1 showing moderate effectiveness, while formulations 2 and 3 showing weak effectiveness. Overall, the study concluded that African leaf extract can be formulated into a hair tonic preparation that meets the standards of physical tests and exhibits antifungal activity against Pityrosporum ovale. These results are expected to contribute to the development of products based on natural ingredients as an alternative to treating fungal infections on the scalp.